Jump to microscope facilities. down
  • DNA-laboratory:
    - Automated Temperature Cycler Heating Blocks for PCR-amplification.
    - Electrophoretic analysis: Power supplies (Consort, BRL).
    - Automated DNA-sequencing equipment (access to Applied Biosystems 3730 DNA Analyzer and 3100 DNA Analyzer at the Dept. Hum.Genetics (seqfac@antrg.umcn.nl)).
    - Incubators/waterbathes, shakers, Eppendorf (mini)centrifuges.
  • Recombinant DNA Safety Room:
    - Biohazard Laminar flow facilities for microorganism handling (Clean Air).
    - Incubators/shaker for microorganism cultivation (New Brunswick G25).
    - High Speed Centrifuge/ Ultracentrifuge (Beckman J2-21).
  • Protein laboratory:
    - Electrophoretic analysis and immunoblotting equipment (Biorad).
    - Equipment for generation and analysis of antibodies.
    - Biochemical cell fractionation.
  • Isotope Laboratory:
    - Equipment necessary for Radioisotope handling.
    - Hybridization incubators.
    - Cell culture for metabolic labeling.
    - 32P Sequencing equipment (Gibco BRL).
  • Cell Culture Rooms:
    - Biohazard Laminar down-flows (Clean Air).
    - Cell culture CO2 incubator (Forma).
    - (Fluorescence-) Research microscopes for cytogenetic analysis.
    - Microinjection facility for production of transgenic mice and embryonic stem cell manipulation: Nikon Diaphot/DIC + Narishige micromanipulation (F.Oerlemans@ncmls.ru.nl).
    - Electroporation equipment (Biorad).
  • Dark room:
    - UV-transilluminator/photography equipment.
    - Developing and print facilities for photos and X-ray film.
  • Storage facilities:
    - Cold Room.
    - Ultra-low -70oC Freezer, Laboratory Freezers, Refrigerators.
up
  • EM- and light-microscopy facilities within the NCMLS-MIC:

    Histology (H.Croes@ncmls.ru.nl):
    - Parafin Embedding Equipment
    - Parafin microtome
    - Cryomicrotome
    Electron Microscopy (J.Fransen@ncmls.ru.nl):
    - Transmission microscopes: JEOL, JEM-1010 and JEM-1210.
    - Scanning Microscope: JEOL SEM6310 with EDS (Tracor Voyager).
    - ultramicrotome (Leica Ultracut S)
    - Cryo-ultramicrotome (Leica UCT with EMFCS).
    - Cryo substitution (Reichert CS auto) and cryo-fixation (Reichert KF80) equipment.
    Time Lapse Microscopy (J.Fransen@ncmls.ru.nl):
    - Nikon Diaphot 300 with CCD camera (Hamamatsu) and Okolab 4 well CO2 stage incubator and Okolab 2D time lapse software
    Fluorescence Microscopy (J.Fransen@ncmls.ru.nl):
    - Zeiss Axioskop.
    - Zeiss Axiophot2 with CCD camera (Axiocam MRm) and Axiovision 3.1 software.
    - Leica DMRA with CCD camera (COHU) and QFluoro 1.2 software.
    Advanced Fluorescence Microscopy (J.Fransen@ncmls.ru.nl):
     - Zeiss Axiovert 200M with Coolsnap HQ CCD camera, Till Photonics Polychrome IV monochromator and Metafluor/Metamorph (Universal Imaging Corporation) image acquisition and analysis software.
    Confocal Microscopy (J.Fransen@ncmls.ru.nl):
    - Biorad MRC1024 with krypton-argon laser.
    - Zeiss LSM 510 meta
    - Noran OZ XL UV-Krypton/argon CLSM with Silicon Graphics Workstation (supervisor W.J.H. Koopman - Dept.Cell Physiology, NCMLS).
    Image Analysis Software (J.Fransen@ncmls.ru.nl):
    - Metamorph/Metafluor offline
    - ImageJ
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